9K iSelect Assay:


[TXT]

9K Assay design file

22-Nov-2011 11:41

4.6M

 


The 9K genotyping assay was described in the following paper:
Cavanagh, C.R., Chao, S., Wang, S., Huang, B.E., Stephen, S., Kiani, S., Forrest, K., Saintenac, C., Brown-Guedira, G.L., Akhunova, A., See, D., Bai, G., Pumphrey, M., Tomar, L., Wong, D., Kong, S., Reynolds, M., da Silva, M.L., Bockelman, H., Talbert, L., et al. (2013). 
Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars. Proc. Natl. Acad. Sci. U. S. A. 110: 8057-62.




90K iSelect Assay:


[TXT]

90K Assay design file

22-Nov-2011 11:41

25M

 

[TXT]

AB_to_nucleotides

22-Mar-2014 11:41

13M

 

[TXT]

90K Consensus Map

22-Jan-2014 12:40

2.5M

 


Genotype calling was performed using the GenomeStudio Polyploid Clustering Module v1.0 Software developed in collaboration with Illumina, Inc.

The design of the wheat 90K iSelect genotyping assay, the development of clustering algorithms and consensus map are described in the following paper:
S. Wang, D. Wong, K. Forrest, A. Allen, S. Chao, E. Huang, M. Maccaferri, S. Salvi, S. Milner, L. Cattivelli, A. M. Mastrangelo, A. Whan, S. Stephen, G. Barker, R. Wieseke, J. Plieske, IWGSC, M. Lillemo, D. Mather, R. Appels, R. Dolferus, G. Brown-Guedira, A. Korol, A. R. Akhunova, C. Feuillet, J. Salse, M. Morgante, C. Pozniak, M. Luo, J. Dvorak, M. Morell, J. Dubcovsky, M. Ganal, R. Tuberosa, C. Lawley, I. Mikoulitch, C. Cavanagh, K. J. Edwards, M. Hayden, E. Akhunov (2014). Characterization of polyploid wheat genomic diversity using a high-density 90,000 SNP array. Plant Biotechnology Journal 2014 Mar 20. doi: 10.1111/pbi.12183. [Epub ahead of print]

For more details, please contact Eduard Akhunov (eakhunov at k-state.edu) or Matt Hayden (matthew.hayden at depi.vic.gov.au).